lb agar plates (Thermo Fisher)

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Thermo Fisher lb agar plates
Lb Agar Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/kanamycin/Kanamycin+monosulfate/pm42176792-240-31-39
Average 94 stars, based on 1 article reviews

lb agar plates - by Bioz Stars, 2026-09

94/100 stars

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Cell Culture:

Article Title: Engineering a Novel Bacterial Encapsulin for Programmable Surface Functionalization: From Single-Target to Mosaic Nanovaccines
Article Snippet: The E. coli codon optimised AmEnc-SpyCatcher (Am-S) sequence ( Table S2 ) was synthesised by Twist Bioscience into the pET-24(+) expression vector and transformed into E. coli BL21(DE3) competent cells (New England Biolabs). .. Cells from a single colony were cultured in a 5 mL starter LB broth culture containing 50 μg/mL of kanamycin (Thermo Fisher Scientific) and grown for 16–18 h at 37 °C with shaking at 200 rpm. ..

Article Title: A versatile plasmid platform for auxotrophic complementation in attenuated Mycobacterium bovis BCG
Article Snippet: .. Escherichia coli strain TOP10 was cultured in Luria–Bertani (LB) medium at 37 °C, with kanamycin (Invitrogen) added to a final concentration of 25 μg/mL when required. .. Mycobacterium bovis BCG ΔLeuD [ ] was grown at 37 °C in Middlebrook 7H9 broth (Difco) supplemented with 10% oleic acid–albumin–dextrose–catalase (OADC; Difco), 0.2% glycerol, and 0.05% Tween 80 (Sigma-Aldrich); or on Middlebrook 7H10 agar (Sigma-Aldrich) supplemented with 10% OADC and 0.2% glycerol.

Concentration Assay:

Article Title: Systematic screening of archaeal MazF homologs reveals Tth-MazF1, a versatile, sequence-specific ribonuclease from Thermococcus thioreducens
Article Snippet: .. The following day, 5 ml of starter culture was used to inoculate 200 ml of Dynamite medium (12 g tryptone, 24 g yeast extract, 6.3 ml glycerol, 3.8 g KH2PO4, 12.5 g K 2 HPO 4 , 5 g glucose, 0.195 g anhydrous MgSO 4 per liter) in a 1 l flask, supplemented with kanamycin at 50 μg/ml (final concentration) and 0.2% (final concentration) arabinose (Thermo Scientific, 365180250). ..

Article Title: Paper-based synthetic gene networks
Article Snippet: .. The concentration for the gene circuits were as follows: FIGS. 16B & 16C: T7_GFP plasmid DNA 5 ng/μl (pBR939b_T7_GFP, Anderson, 2007); FIG. 16E: PN25-GFP plasmid DNA 30 ng/μl and T7_GFP plasmid DNA 30 ng/μl; FIG. 16G: Tet-O GFP and TetO-mCherry plasmid DNA 30 ng/μl; FIG. 17: linear DNA 33 nM, RNA triggers at 5 tM; FIG. 18: linear DNA 33 nM, RNA triggers at 5 μM or as specified; FIGS. 19B & 19C: mCherry and GFP mRNA sensors linear DNA 33 nM, respective mRNAs at 2.5 tM; FIGS. 16D & 16E: antibiotic resistance gene mRNA sensors for spectinomycin, chloramphenicol, kanamycin and ampicillin linear DNA 33 nM, respective mRNAs at 3 μM or as specified; FIG. 19F: pT7CFE1_GFP plasmid DNA (Thermo Scientific) 30 ng/μl, pcDNA3.1 FLIPglu-30uDeltal3V plasmid DNA (Takanaga 2010, addgene:18015) 30 ng/μl; FIG. 39: PCR product was not gel-purified and therefore used a 5×linear DNA concentration of 150 ng/μl to ensure adequate sensor product, respective trigger RNAs at 3 μM or as specified; FIG. 40: T3 and T7 cascade modules plasmid DNA 30 ng/μl (E. coli RNAP_trigger 1, Ecoli RNAP_switch_1_T3RNAP, E. coli RNAP_trigger 2, E. coli RNAP_switch_2_T7RNAP), T3_GFP and T7_GFP plasmid DNA 40 ng/μl, T3 RNAP_trigger_3 and T7 RNAP_switch 3_GFP plasmid DNA 40 ng/μl. ..

Incubation:

Article Title: Multimodal control of Cas13d activity through domain insertion at an allosteric hotspot.
Article Snippet: The catalytically dead d fxCas13d was generated by sitedirected mutagenesis (Phusion High-Fidelity DNA-Polymerase, ThermoFisher). .. On a 96 well microtiter plate (96F clear plate, ThermoFisher) 200μL LB with streptomycin and kanamycin was inoculated with a single colony, the plate sealed with a breathable film (BF-410400-S, Corning) and incubated at 30°C for 24 hours with agitation. .. LightR-dCasRx was generated by Gibson Assembly, with the E. coli codon-optimized LightR sequence (synthesized by GeneArt, Regensburg) as template for the insert.

Article Title: Mechanism of K63-linked polyubiquitin recognition and cleavage by the BRCA1-A complex
Article Snippet: .. Bacterial cells were grown in Luria-Bertani (LB) broth (Fisher Chemical) or Terrific Broth (TB; Fisher Chemical) media supplemented with appropriate antibiotics [100 μg/mL ampicillin (Merk Life Science), 34 μg/mL chloramphenicol (Amresco), 10 μg/mL gentamycin (VWR), 50 μg/mL kanamycin (Thermo Fisher Scientific), 10 μg/mL tetracycline (Sigma)], and incubated at 37°C and 18°C as per requirements. .. For preparation of bacmids DNA, x-gal (Thermo Fisher Scientific) and isopropyl-β-D-1-thiogalactopyranoside (IPTG; Fluorochem) were also added to bacteria cultures at final concentrations of 15 μg/mL and 40 μg/mL, respectively.

Plasmid Preparation:

Polymerase Chain Reaction:

other:

Article Title: A catabolic powerhouse for biorefineries: characterization and engineering Erwinia spp. strain LJJL01 to produce bioproducts.
Article Snippet: Antibiotic tolerance assay The concentrations of antibiotics tested in this study were 25, 50, 100 and 150 μg/mL kanamycin (Alfa Aesar, USA) 50, 100, 200, and 500 μg/mL ampicillin (Alfa Aesar, USA), 12.5, 25, 50 and 125 μg/mL chloramphenicol (Gold Biotechnology, USA), 5, 10, 20 and μg/mL tetracycline (Alfa Aesar, USA) and 50, 100, 200, and 500 μg/mL spectinomycin (Gold Biotechnology, USA).

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